MERTK Polyklonaler Antikörper

MERTK Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 27900-1-AP

Synonyme

c mer, EC:2.7.10.1, MER, Proto oncogene c Mer, Proto-oncogene c-Mer

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  WB analysis using 27900-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27900-1-AP (MERTK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 27900-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27900-1-AP (MERTK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 27900-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27900-1-AP (MERTK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 27900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 27900-1-AP (MERTK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Jurkat using 27900-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MERTK Polyclonal antibody (27900-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, Maushodengewebe, U-937-Zellen
• Erfolgreiche Detektion in IHC: Rattenlebergewebe, humanes Lungengewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

27900-1-AP bindet in WB, IHC, IF, FC (Intra), ELISA MERTK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MERTK fusion protein Ag27540
• Vollständiger Name: c-mer proto-oncogene tyrosine kinase
• Berechnetes Molekulargewicht: 999 aa, 110 kDa
• Beobachtetes Molekulargewicht: 160-180 kDa
• GenBank-Zugangsnummer: BC114918
• Gene symbol: MERTK
• Gene ID (NCBI): 10461
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MerTK (Mer tyrosine kinase), also known as RP38, c-Eyk, c-mer, and Tyro12, was first cloned from a human B lymphoblastoid expression library (PMID:8086340) and is one of the TAM (Tyro-3, Axl, and MerTK) receptor tyrosine kinase (RTK) family (PMID:23833304). Although this RTK, like others, can promote tumor cell proliferation to some extent, MERTK primarily lends tumor cells crucial survival advantages while promoting invasion, migration and metastasis, drug resistance, and, in the innate immune system, suppressing anti-tumor immunity (PMID:32417270). The multiple MERTK species observed are likely due to posttranslational modifications (PMID: 23585477).

Publikationen

Species	Application	Title
human	IF	Cell Rep Med; Disruption of MerTK increases the efficacy of checkpoint inhibitor by enhancing ferroptosis and immune response in hepatocellular carcinoma; Authors - Shun Wang
human	IHC	Eur Arch Otorhinolaryngol; Aberrant expressions of TAM receptors are associated with postoperative recurrence in chronic rhinosinusitis with nasal polyps; Authors - Yandan Zhou
human	WB	Front Endocrinol (Lausanne); Downregulation of MerTK in circulating T cells of patients with non-proliferative diabetic retinopathy; Authors - Shimiao Bu
mouse	WB	J Ethnopharmacol; Longan Aril polysaccharides ameliorate cognitive impairment in AD mice via restoration of the immune phagocytosis of microglia; Authors - Han Zhang
mouse	WB	J Ethnopharmacol; Xin-shu-bao tablets ameliorates ventricular remodeling against HFrEF via PPARγ/MFGE8 pathway based on MALDI-MSI and Lipidomics; Authors - Fengrong Zhang