MERTK Rekombinanter Antikörper

MERTK Rekombinant Antikörper für WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241252H3

Kat-Nr. : 84156-2-PBS

Synonyme

241252H3, c mer, EC:2.7.10.1, MER, Proto oncogene c Mer

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  WB analysis using 84156-2-RR (same clone as 84156-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84156-2-RR (MERTK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84156-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01061-2

  Sandwich ELISA standard curve of MP01061-2, Human MERTK Recombinant Matched Antibody Pair - PBS only. 84156-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27540. 84156-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP01061-2

  The mean MERTK concentration was determined to be 5,583.9 pg/mL in HepG2 cell extract based on a 2.3 mg/mL extract load.

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  IHC staining of mouse kidney using 84156-2-RR (same clone as 84156-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84156-2-RR (MERTK antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84156-2-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 84156-2-RR (same clone as 84156-2-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MERTK Recombinant antibody (84156-2-RR, Clone:241252H3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84156-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84156-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84156-2-RR against Human MERTK were performed. The affinity constant is 0.444 nM.

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Geprüfte Anwendungen

Produktinformation

84156-2-PBS bindet in WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test MERTK und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MERTK fusion protein Ag27540
• Vollständiger Name: c-mer proto-oncogene tyrosine kinase
• Berechnetes Molekulargewicht: 999 aa, 110 kDa
• Beobachtetes Molekulargewicht: 160-180 kDa
• GenBank-Zugangsnummer: BC114918
• Gene symbol: MERTK
• Gene ID (NCBI): 10461
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MerTK (Mer tyrosine kinase), also known as RP38, c-Eyk, c-mer, and Tyro12, was first cloned from a human B lymphoblastoid expression library (PMID:8086340) and is one of the TAM (Tyro-3, Axl, and MerTK) receptor tyrosine kinase (RTK) family (PMID:23833304). Although this RTK, like others, can promote tumor cell proliferation to some extent, MERTK primarily lends tumor cells crucial survival advantages while promoting invasion, migration and metastasis, drug resistance, and, in the innate immune system, suppressing anti-tumor immunity (PMID:32417270). The multiple MERTK species observed are likely due to posttranslational modifications (PMID: 23585477).