MEST Polyklonaler Antikörper

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• KD/KO Validated

MEST Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 11118-1-AP

Synonyme

MEST, PEG1

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Galerie der Validierungsdaten

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  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11118-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11118-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 11118-1-AP

  IP Result of anti-MEST (IP:11118-1-AP, 3ug; Detection:11118-1-AP 1:500) with PC-3 cells lysate 1720ug.

• 

  IHC staining of human pancreas cancer using 11118-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11118-1-AP (MEST antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11118-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11118-1-AP (MEST antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Maushodengewebe, PC-3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

11118-1-AP bindet in WB, IP, IHC, IF, ELISA MEST und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MEST fusion protein Ag1617
• Vollständiger Name: mesoderm specific transcript homolog (mouse)
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC011908
• Gene symbol: MEST
• Gene ID (NCBI): 4232
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene, which is associated with growth of mesodermal origin cells(PMID:20339302) and plays important roles in embryo development(PMID:21375506). It is also a novel regulator of Wnt/CTNNB signalling during adipogenic differentiation(PMID:21375506). It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
mouse	IHC	Nucleic Acids Res; Tissue-specific alternative polyadenylation at the imprinted gene Mest regulates allelic usage at Copg2.; Authors - Julia L MacIsaac
human	IHC	Gynecol Oncol; Increased co-expression of MEST and BRCA1 is associated with worse prognosis and immune infiltration in ovarian cancer.; Authors - Jing Zhang
human	WB	Cancer Lett; Novel target genes responsive to the anti-growth activity of triptolide in endometrial and ovarian cancer cells.; Authors - Li Haili H
human	WB,IHC	Placenta; DNA methylation-associated repression of MEST/PEG1 expression contributes to the invasion of extravillous trophoblast cells.; Authors - Wei Peng ; KD Validated
human	WB	Placenta; Maternal obesity and gestational weight gain are modestly associated with umbilical cord DNA methylation.; Authors - Keshari M Thakali