METTL1 Polyklonaler Antikörper

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• KD/KO Validated

METTL1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14994-1-PBS

Synonyme

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  WB analysis using 14994-1-AP (same clone as 14994-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14994-1-AP (same clone as 14994-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

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  IP experiment of A2780 using 14994-1-AP (same clone as 14994-1-PBS)

  IP result of anti-METTL1 (IP:14994-1-AP, 4ug; Detection:14994-1-AP 1:300) with A2780 cells lysate 3600 ug. This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14994-1-AP (same clone as 14994-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14994-1-AP (METTL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14994-1-AP (same clone as 14994-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14994-1-AP (METTL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14994-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14994-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA METTL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: METTL1 fusion protein Ag6980
• Vollständiger Name: methyltransferase like 1
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC000550
• Gene symbol: METTL1
• Gene ID (NCBI): 4234
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.