METTL13 Polyklonaler Antikörper

METTL13 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24379-1-AP

Synonyme

CGI 01, EEF1AKNMT, FEAT, KIAA0859, methyltransferase like 13

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  WB analysis using 24379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24379-1-AP (METTL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 24379-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using METTL13 antibody (24379-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 5637 cells
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24379-1-AP bindet in WB, IF/ICC, ELISA METTL13 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: METTL13 fusion protein Ag19355
• Vollständiger Name: methyltransferase like 13
• Berechnetes Molekulargewicht: 699 aa, 79 kDa
• Beobachtetes Molekulargewicht: 72-78 kDa
• GenBank-Zugangsnummer: BC029083
• Gene symbol: METTL13
• Gene ID (NCBI): 51603
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein methyltransferase like 13 (METTL13), also called FEAT, is coded by gene METTL13, which is located at chromosome 1q24.3. Purified from rat livers in 2011 by a Japanese researcher, METTL13 was found to be abnormally overexpressed in several human cancers including lung cancer and to drive tumorigenesis in transgenic mice. A study indicated that METTL13 inhibits apoptosis of lung, breast and liver cancer cells and miR-16 can repress the expression of METTL13 by binding to its 3′-untranslated region. (PMID: 33985542)