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METTL2 Polyklonaler Antikörper

METTL2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16983-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using METTL2 Polyclonal antibody (16983-1-AP)

    WB analysis using 16983-1-AP (same clone as 16983-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16983-1-AP (METTL2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using METTL2 Polyclonal antibody (16983-1-AP)

    IHC staining of mouse colon using 16983-1-AP (same clone as 16983-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16983-1-AP (METTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using METTL2 Polyclonal antibody (16983-1-AP)

    IHC staining of mouse colon using 16983-1-AP (same clone as 16983-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16983-1-AP (METTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using METTL2 Polyclonal antibody (16983-1-AP)

    IF Staining of MCF-7 using 16983-1-AP (same clone as 16983-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL2 antibody (16983-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

16983-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA METTL2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen METTL2 fusion protein Ag10410
Vollständiger Name methyltransferase like 2B
Berechnetes Molekulargewicht 313 aa, 36 kDa
Beobachtetes Molekulargewicht36 kDa
GenBank-ZugangsnummerBC107586
Gene symbol METTL2B
Gene ID (NCBI) 55798
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.