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METTL2 Rekombinanter Antikörper

METTL2 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241490A3

Kat-Nr. : 84257-1-PBS

Synonyme

METTL2B, 241490A3, EC:2.1.1.-, methyltransferase like 2B, Methyltransferase-like protein 2B

Filter:
  • Western Blot (WB) analysis of HeLa cells using METTL2 Recombinant antibody (84257-1-RR)

    WB analysis of HeLa using 84257-1-RR (same clone as 84257-1-PBS)

    WB result of METTL2 antibody (84257-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL2 transfected HeLa cells. This data was developed using the same antibody clone with 84257-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using METTL2 Recombinant antibody (84257-1-RR)

    WB analysis using 84257-1-RR (same clone as 84257-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84257-1-RR (METTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84257-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using METTL2 Recombinant antibody (84257-1-RR)

    IF Staining of MCF-7 using 84257-1-RR (same clone as 84257-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL2 antibody (84257-1-RR, Clone: 241490A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84257-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01163-3

    Cytometric bead array standard curve of MP01163-3

    Cytometric bead array standard curve of MP01163-3, METTL2B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84257-1-PBS. Detection antibody: 84257-3-PBS. Standard: Ag10410. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84257-1-RR

    Affinity and Kinetic Characterization of 84257-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84257-1-RR against Human METTL2 were performed. The affinity constant is 0.741 nM.

Geprüfte Anwendungen

Produktinformation

84257-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA METTL2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen METTL2 fusion protein Ag10410
Vollständiger Name methyltransferase like 2B
Berechnetes Molekulargewicht 313 aa, 36 kDa
Beobachtetes Molekulargewicht36~43 kDa
GenBank-ZugangsnummerBC107586
Gene symbol METTL2B
Gene ID (NCBI) 55798
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.