METTL2 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

METTL2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241490A3

Kat-Nr. : 84257-1-RR

Synonyme

METTL2B, 241490A3, EC:2.1.1.-, methyltransferase like 2B, Methyltransferase-like protein 2B

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  WB analysis of HeLa using 84257-1-RR

  WB result of METTL2 antibody (84257-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL2 transfected HeLa cells.

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  WB analysis using 84257-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84257-1-RR (METTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 84257-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL2 antibody (84257-1-RR, Clone: 241490A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84257-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84257-1-RR against Human METTL2 were performed. The affinity constant is 0.741 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, MCF-7-Zellen, PC-3-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84257-1-RR bindet in WB, IF/ICC, ELISA METTL2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: METTL2 fusion protein Ag10410
• Vollständiger Name: methyltransferase like 2B
• Berechnetes Molekulargewicht: 313 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36~43 kDa
• GenBank-Zugangsnummer: BC107586
• Gene symbol: METTL2B
• Gene ID (NCBI): 55798
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.