METTL3 Monoklonaler Antikörper

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• KD/KO Validated

METTL3 Monoklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

CloneNo.

2D8H1

Kat-Nr. : CL594-67733

Synonyme

2D8H1, EC:2.1.1.348, hMETTL3, Methyltransferase-like protein 3, MT A70

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  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:400.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:400.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

• 

  FC experiment of HEK-293 using CL594-67733

  1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human METTL3 (CL594-67733, Clone:2D8H1) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushodengewebe
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67733 bindet in IF-P, FC (Intra) METTL3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: METTL3 fusion protein Ag7110
• Vollständiger Name: methyltransferase like 3
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC001650
• Gene symbol: METTL3
• Gene ID (NCBI): 56339
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Publikationen

Species	Application	Title
mouse	FC (Intra)	BMC Med; METTL3 facilitates kidney injury through promoting IRF4-mediated plasma cell infiltration via an m6A-dependent manner in systemic lupus erythematosus; Authors - Yu Liu