METTL5 Polyklonaler Antikörper

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• KD/KO Validated

METTL5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16791-1-PBS

Synonyme

EC:2.1.1.-, HSPC133, methyltransferase like 5, Methyltransferase-like protein 5, rRNA N6-adenosine-methyltransferase METTL5

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  WB analysis of K-562 using 16791-1-AP (same clone as 16791-1-PBS)

  WB result of METTL5 antibody (16791-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected K-562 cells. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

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  WB analysis using 16791-1-AP (same clone as 16791-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16791-1-AP (METTL5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16791-1-AP (same clone as 16791-1-PBS)

  WB result of METTL5 antibody (16791-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected HeLa cells. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16791-1-AP (same clone as 16791-1-PBS)

  WB result of METTL5 antibody (16791-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected HeLa cells. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 16791-1-AP (same clone as 16791-1-PBS)

  IP result of anti-METTL5 (IP:16791-1-AP, 4ug; Detection:16791-1-AP 1:500) with PC-3 cells lysate 3840 ug. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 16791-1-AP (same clone as 16791-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 16791-1-AP (same clone as 16791-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 16791-1-AP (same clone as 16791-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16791-1-AP (same clone as 16791-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using METTL5 antibody (16791-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16791-1-AP (same clone as 16791-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using METTL5 antibody (16791-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16791-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA METTL5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: METTL5 fusion protein Ag10232
• Vollständiger Name: methyltransferase like 5
• Berechnetes Molekulargewicht: 209 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24-27 kDa
• GenBank-Zugangsnummer: BC000921
• Gene symbol: METTL5
• Gene ID (NCBI): 29081
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm.