METTL6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

METTL6 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 16527-1-AP

Synonyme

EC:2.1.1.-, hMETTL6, methyltransferase like 6, Methyltransferase-like protein 6, tRNA N(3)-methylcytidine methyltransferase METTL6

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  WB analysis using 16527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16527-1-AP (METTL6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16527-1-AP (METTL6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 16527-1-AP

  IP result of anti-METTL6 (IP:16527-1-AP, 4ug; Detection:16527-1-AP 1:5000) with MCF-7 cells lysate 1600 ug.

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  IHC staining of human lung cancer using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16527-1-AP (METTL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16527-1-AP (METTL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Hirngewebe, humanes Mammakarzinomgewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

16527-1-AP bindet in WB, IHC, IP, ELISA METTL6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: METTL6 fusion protein Ag9768
• Vollständiger Name: methyltransferase like 6
• Berechnetes Molekulargewicht: 255 aa, 30 kDa
• Beobachtetes Molekulargewicht: 30-32 kDa
• GenBank-Zugangsnummer: BC022400
• Gene symbol: METTL6
• Gene ID (NCBI): 131965
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent non tumor tissues. CRISPR/Cas9 mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).

Publikationen

Species	Application	Title
human	IHC	Front Immunol; Identification and validation of a tyrosine metabolism-related prognostic prediction model and characterization of the tumor microenvironment infiltration in hepatocellular carcinoma; Authors - Yangying Zhou
human	WB	J Biol Chem; Three distinct 3-methylcytidine (m(3)C) methyltransferases modify tRNA and mRNA in mice and humans.; Authors - Luang Xu ; KO Validated
human	WB	Nat Commun; m3C32 tRNA modification controls serine codon-biased mRNA translation, cell cycle, and DNA-damage response; Authors - Jia Cui ; KO Validated