METTL6 Rekombinanter Antikörper

METTL6 Rekombinant Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241482F9

Kat-Nr. : 84209-5-PBS

Synonyme

241482F9, EC:2.1.1.-, hMETTL6, methyltransferase like 6, Methyltransferase-like protein 6

---

• 

  IHC staining of mouse testis using 84209-5-RR (same clone as 84209-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84209-5-RR (METTL6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84209-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84209-5-RR (same clone as 84209-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using METTL6 antibody (84209-5-RR, Clone: 241482F9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84209-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84209-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84209-5-RR against Human METTL6 were performed. The affinity constant is 7.69 nM.

---

Geprüfte Anwendungen

Produktinformation

84209-5-PBS bindet in IHC, IF/ICC, Indirect ELISA METTL6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: METTL6 fusion protein Ag9714
• Vollständiger Name: methyltransferase like 6
• Berechnetes Molekulargewicht: 255 aa, 30 kDa
• GenBank-Zugangsnummer: BC022400
• Gene symbol: METTL6
• Gene ID (NCBI): 131965
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent non tumor tissues. CRISPR/Cas9 mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).