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METTL8 Polyklonaler Antikörper

METTL8 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31173-1-PBS

Synonyme

methyltransferase like 8

Filter:
  • Western Blot (WB) analysis of various lysates using METTL8 Polyclonal antibody (31173-1-AP)

    WB analysis using 31173-1-AP (same clone as 31173-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31173-1-AP (METTL8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31173-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using METTL8 Polyclonal antibody (31173-1-AP)

    IF Staining of A431 using 31173-1-AP (same clone as 31173-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using METTL8 antibody (31173-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31173-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

31173-1-PBS bindet in WB, IF/ICC, Indirect ELISA METTL8 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen METTL8 fusion protein Ag34222
Vollständiger Name methyltransferase like 8
Berechnetes Molekulargewicht33 kDa
Beobachtetes Molekulargewicht33~40 kDa
GenBank-ZugangsnummerBC025250
Gene symbol METTL8
Gene ID (NCBI) 79828
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

METTL8 (methyltransferase-like protein 8) is an m3C methyltransferase that is best known for its mitochondrial role in installing m3C32 on mt-tRNAThr/Ser(UCN). METTL8 has proved to be a transcriptional target of STAT3 that mediates STAT3's functions in mESCs (PMID: 29706498). METTL8 has multiple splicing isoforms and can undergo sumoylation and associate with nuclear RNA-binding proteins to regulate R-loop formation on ribosomal DNA gene (presumably via its methyltransferase activity on m3C) (PMID: 38744809).