METTL9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

METTL9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 15120-1-AP

Synonyme

CGI 81, CGI81, CGI-81, DREV, DREV1

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• 

  WB analysis using 15120-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15120-1-AP (METTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15120-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15120-1-AP (METTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15120-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15120-1-AP (METTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15120-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15120-1-AP (METTL9 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15120-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15120-1-AP (METTL9 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 15120-1-AP

  Immunofluorescent analysis of HepG2 cells using 15120-1-AP (METTL9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

15120-1-AP bindet in WB, IHC, IF/ICC, ELISA METTL9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: METTL9 fusion protein Ag7191
• Vollständiger Name: methyltransferase like 9
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC000195
• Gene symbol: METTL9
• Gene ID (NCBI): 51108
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

METTL9 is a broad-specificity methyltransferase that mediates the formation of the majority of 1-methylhistidine(1MH) present in mouse and human proteomes.

Publikationen

Species	Application	Title
human	WB	Biochem Biophys Rep; Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers.; Authors - Toshifumi Hara ; KD Validated
human	WB,IHC	Cell Death Discov; METTL9-SLC7A11 axis promotes hepatocellular carcinoma progression through ferroptosis inhibition; Authors - Fangfang Bi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhongwen (Verified Customer) (09-25-2023)

Low quality, cannot find the target band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

METTL9 Antibody Western Blot validation (1:1000 dilution) in (Cat no:15120-1-AP)