MFAP4 Polyklonaler Antikörper

MFAP4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 17661-1-AP

Synonyme

Microfibril-associated glycoprotein 4

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  WB analysis of human kidney using 17661-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 17661-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse heart using 17661-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 17661-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17661-1-AP (MFAP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, Mausherzgewebe, Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

17661-1-AP bindet in WB, IHC, IF, ELISA MFAP4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MFAP4 fusion protein Ag11920
• Vollständiger Name: microfibrillar-associated protein 4
• Berechnetes Molekulargewicht: 255 aa, 29 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC062415
• Gene symbol: MFAP4
• Gene ID (NCBI): 4239
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Microfibrillar-associated protein 4 (MFAP4) is a extracellular matrix glycoprotein containing a fibrinogen C-terminal domain and a N-terminal located integrin-binding motif. It could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 gene is commonly deleted in Smith-Magenis syndrome patients. (PMID: 7633408; 16867155; 10542261; 18322703)

Publikationen

Species	Application	Title
human	WB,IHC	Thorax; A large lung gene expression study identifying fibulin-5 as a novel player in tissue repair in COPD.; Authors - Corry-Anke Brandsma
human	WB	Front Pharmacol; MFAP4 is a novel prognostic biomarker in glioma correlating with immunotherapy resistance and ferroptosis; Authors - Yuanhao Lv
human	WB	J Proteome Res; Strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples.; Authors - Muraoka Satoshi S
human	IHC,IF	FASEB J; The "Matrisome" reveals the characterization of skin keloid microenvironment.; Authors - Shikun Zhang
mouse	WB	J Proteomics; FOXL1 + Telocytes in mouse colon orchestrate extracellular matrix biodynamics and wound repair resolution; Authors - Veronique Pomerleau
mouse	WB,IF	BMC Biol; The m6A writer KIAA1429 regulates photoaging progression via MFAP4-dependent collagen synthesis; Authors - Yuanyuan Liu