MFN1 Monoklonaler Antikörper

MFN1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(26)

CloneNo.

3F11C11

Rezensionen (2)

Kat-Nr. : 66776-1-Ig

Synonyme

3F11C11, EC:3.6.5.-, Fzo homolog, hfzo1, hfzo2

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  WB analysis using 66776-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66776-1-Ig (MFN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66776-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66776-1-Ig (MFN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 66776-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66776-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66776-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66776-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66776-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFN1 antibody (66776-1-Ig, Clone: 3F11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-3-Zellen, U-251-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Produktinformation

66776-1-Ig bindet in WB, IHC, IF/ICC, ELISA MFN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MFN1 fusion protein Ag4890
• Vollständiger Name: mitofusin 1
• Berechnetes Molekulargewicht: 741 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC040557
• Gene symbol: MFN1
• Gene ID (NCBI): 55669
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

Publikationen

Species	Application	Title
human	IF	Nat Commun; BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects.; Authors - Gee Euhn Choi
pig	IF	Ecotoxicol Environ Saf; Zinc deficiency compromises the maturational competence of porcine oocyte by inducing mitophagy and apoptosis; Authors - Xin-Le Lai
pig	IF	J Cell Physiol; DMT1 Maintains Iron Homeostasis to Regulate Mitochondrial Function in Porcine Oocytes; Authors - Jin-Xin Zhang
human	WB	Molecules; Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy.; Authors - Jiling Feng
human	WB	Appl Biochem Biotechnol; Astragaloside IV Relieves Mitochondrial Oxidative Stress Damage and Dysfunction in Diabetic Mice Endothelial Progenitor Cells by Regulating the GSK-3β/Nrf2 Axis; Authors - Xiaoling Zou
Human		J Membr Biol; Schisandrin B Alleviates LPS Induced Mitochondrial Damage in C28I2 Cells; Authors - Fei Hu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Pierre (Verified Customer) (09-26-2025)

Excellent

• Applications: Western Blot
• Cell Tissue Type: T cells

FH

benjamin (Verified Customer) (01-31-2019)

Very strong for WB with 20ug of protein using heLa cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa PWPI