MFN2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MFN2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (7)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(460)

Rezensionen (4)

Kat-Nr. : 12186-1-AP

Synonyme

CPRP1, EC:3.6.5.-, HSG, KIAA0214, MARF

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  WB analysis using 12186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12186-1-AP (MFN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12186-1-AP (MFN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12186-1-AP

  WB result of MFN2 antibody (12186-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MFN2 transfected HeLa cells.

• 

  WB analysis using 12186-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12186-1-AP (MFN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12186-1-AP

  IP result of anti-MFN2 (IP:12186-1-AP, 4ug; Detection:12186-1-AP 1:10000) with mouse kidney tissue lysate 2160 ug.

• 

  IHC staining of human colon cancer using 12186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12186-1-AP (MFN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12186-1-AP (MFN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12186-1-AP (MFN2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Mausnierengewebe, Mauslebergewebe, Rattenhirngewebe, Rattenherzgewebe, Rattennierengewebe, Rattenlebergewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 18 publications below
• WB: See 437 publications below
• IHC: See 29 publications below
• IF: See 39 publications below
• IP: See 8 publications below
• CoIP: See 4 publications below

Produktinformation

12186-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA MFN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Ente, Hausschwein, Huhn, Maus, Ratte, Rind, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MFN2 fusion protein Ag2845
• Vollständiger Name: mitofusin 2
• Berechnetes Molekulargewicht: 757 aa, 86 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC017061
• Gene symbol: MFN2
• Gene ID (NCBI): 9927
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.

Publikationen

Species	Application	Title
mouse	WB	Nature; Cellular ATP demand creates metabolically distinct subpopulations of mitochondria; Authors - Keun Woo Ryu ; KO Validated
mouse	WB	Cell; Mitocytosis, a migrasome-mediated mitochondrial quality-control process.; Authors - Haifeng Jiao ; KD Validated
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
mouse	WB	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
mouse,human	WB,IF	Nat Commun; Engineered hypoxia-responsive albumin nanoparticles mediating mitophagy regulation for cancer therapy; Authors - Wenyan Wang
mouse	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (09-23-2024)

Good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

MFN2 Antibody Western Blot validation (1000 dilution) in A549 (Cat no:12186-1-AP)

FH

Nin (Verified Customer) (02-27-2023)

A reliable Ab to detect endogenous Mfn2

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain lysate

MFN2 Antibody Western Blot validation (1:3000 dilution) in mouse brain lysate (Cat no:12186-1-AP)

FH

Mi (Verified Customer) (02-21-2023)

This antibody works pretty well in adipocytes, we got a single and clean band at the expected size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes

FH

Julia (Verified Customer) (11-16-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293 cells