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MFN2 Monoklonaler Antikörper

MFN2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

CloneNo.

5F3B3

Kat-Nr. : 67487-1-Ig

Synonyme

5F3B3, CPRP1, EC:3.6.5.-, HSG, KIAA0214



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen
Erfolgreiche Detektion in IHChumanes Herzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

67487-1-Ig bindet in WB, IHC, IF/ICC, ELISA MFN2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Fisch, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen MFN2 fusion protein Ag29873
Vollständiger Name mitofusin 2
Berechnetes Molekulargewicht 757 aa, 86 kDa
Beobachtetes Molekulargewicht 86 kDa
GenBank-ZugangsnummerBC017061
Gene symbol MFN2
Gene ID (NCBI) 9927
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for MFN2 antibody 67487-1-IgProtokoll herunterladen
IHC protocol for MFN2 antibody 67487-1-IgProtokoll herunterladenl
IF protocol for MFN2 antibody 67487-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

iScience

MFN2 coordinates mitochondria motility with α-tubulin acetylation and this regulation is disrupted in CMT2A

Authors - Atul Kumar
  • KD Validated
humanWB

Int Immunopharmacol

IDH1/MDH1 deacetylation promotes NETosis by regulating OPA1 and autophagy

Authors - Yukun Wang
HumanWB

Front Pharmacol

Mitochonic Acid-5 Inhibits Reactive Oxygen Species Production and Improves Human Chondrocyte Survival by Upregulating SIRT3-Mediated, Parkin-dependent Mitophagy.

Authors - Ruobing Xin
humanWB

Molecules

Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy.

Authors - Jiling Feng
fishWB

J Physiol Sci

Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart

Authors - Zhanglin Chen
Human

J Membr Biol

Schisandrin B Alleviates LPS Induced Mitochondrial Damage in C28I2 Cells

Authors - Fei Hu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Pierre (Verified Customer) (09-26-2025)

Very good

  • Applications: Western Blot
  • Cell Tissue Type: T cells