MFN2 Rekombinanter Antikörper

MFN2 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A5

Kat-Nr. : 82673-2-PBS

Synonyme

MARF, KIAA0214, HSG, EC:3.6.5.-, CPRP1

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  WB analysis using 82673-2-RR (same clone as 82673-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82673-2-RR (MFN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82673-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82673-2-RR (same clone as 82673-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82673-2-RR (MFN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82673-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82673-2-RR (same clone as 82673-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82673-2-RR (MFN2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82673-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82673-2-RR (same clone as 82673-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MFN2 antibody (82673-2-RR, Clone: 2A5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82673-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82673-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82673-2-RR against Human MFN2 were performed. The affinity constant is 19.7 nM.

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Geprüfte Anwendungen

Produktinformation

82673-2-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MFN2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MFN2 fusion protein Ag2845
• Vollständiger Name: mitofusin 2
• Berechnetes Molekulargewicht: 757 aa, 86 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC017061
• Gene symbol: MFN2
• Gene ID (NCBI): 9927
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitofusin-2 (MFN2), a mitochondrial membrane protein, is a dynamin-like GTPase embedded in the outer mitochondrial membrane (OMM) which plays a central role in regulating mitochondrial fusion and cell metabolism (PMID: 35399520; 34026850). There are two human mitofusin isoforms MFN1 and MFN2, MFN1 and MFN2 mediate outer membrane fusion, OPA1 is involved in inner membrane fusion, and DRP1 is responsible for mitochondrial fission (PMID: 17084350; 35399520). It has been shown that MFN2 participates in multiple cellular biological processes under physical and pathological conditions. Apart from mitochondria fusion, MFN2 is also involved in mitochondrial-ER contacts (MERCs), mitophagy, cellular apoptosis, and proliferation (PMID: 34026850).