MFSD2 Polyklonaler Antikörper

MFSD2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 25498-1-AP

Synonyme

FP1147, HMFN0656, MFSD2, MFSD2A, PP10484, PP9177, RP3 342P20.1, UNQ300/PRO341

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  WB analysis of HepG2 using 25498-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25498-1-AP (MFSD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 25498-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25498-1-AP (MFSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 25498-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25498-1-AP (MFSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25498-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25498-1-AP (MFSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25498-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25498-1-AP (MFSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 25498-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25498-1-AP (MFSD2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 25498-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25498-1-AP (MFSD2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hodengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

25498-1-AP bindet in WB, IHC, IF, ELISA MFSD2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MFSD2 fusion protein Ag22232
• Vollständiger Name: major facilitator superfamily domain containing 2
• Berechnetes Molekulargewicht: 543 aa, 60 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC092414
• Gene symbol: MFSD2
• Gene ID (NCBI): 84879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MFSD2, also named as MFSD2A, belongs to a large family of presumptive carbohydrate transporters with 10-12 membrane-spanning domains, is located at chromosomal position 1p34.2, and is conserved in evolution. It plays a role in thermogenesis via beta-adrenergic signaling pathway. It may be the main plasma membrane tunicamycin transporter. MFSD2 has three isoforms with MW 50 kDa and 59-60 kDa.

Publikationen

Species	Application	Title
mouse	WB,IHC,IF	Neuro Oncol; Wnt signaling regulates MFSD2A-dependent drug delivery through endothelial transcytosis in glioma; Authors - Yuan Xie
human	WB	bioRxiv; Modulation of blood-tumor barrier transcriptional programs improves intra-tumoral drug delivery and potentiates chemotherapy in GBM; Authors - Jorge L Jimenez-Macias
human	WB	Sci Adv; Modulation of blood-tumor barrier transcriptional programs improves intratumoral drug delivery and potentiates chemotherapy in GBM; Authors - Jorge L Jimenez-Macias