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MGAT3 Polyklonaler Antikörper

MGAT3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17869-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of COLO 320 cells using MGAT3 Polyclonal antibody (17869-1-AP)

    WB analysis of COLO 320 using 17869-1-AP (same clone as 17869-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17869-1-AP (MGAT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using MGAT3 Polyclonal antibody (17869-1-AP)

    WB analysis of Caco-2 using 17869-1-AP (same clone as 17869-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 17869-1-AP (MGAT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MGAT3 Polyclonal antibody (17869-1-AP)

    IHC staining of human colon cancer using 17869-1-AP (same clone as 17869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17869-1-AP (MGAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MGAT3 Polyclonal antibody (17869-1-AP)

    IHC staining of human colon cancer using 17869-1-AP (same clone as 17869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17869-1-AP (MGAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

17869-1-PBS bindet in WB, IHC, Indirect ELISA MGAT3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MGAT3 fusion protein Ag12352
Vollständiger Name mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
Berechnetes Molekulargewicht 533 aa, 61 kDa
Beobachtetes Molekulargewicht 61 kDa
GenBank-ZugangsnummerBC075026
Gene symbol MGAT3
Gene ID (NCBI) 4248
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In the small intestine, ~75% of triacylglycerol (TG) synthesis occurs via the monoacylglycerol acyltransferase (MGAT) pathway.Three MGAT isoforms (MGAT1, MGAT2 and MGAT3) have been identifed based on their extensive homology to DGAT2. MGAT1 is primarily expressed in the adipose tissue, stomach and kidney but not in the small intestine. MGAT2 and MGAT3 are most highly expressed in the small intestine, suggesting that these two MGAT forms are responsible for the resynthesis of dietary TG that is packaged into chylomicrons.