MGAT4C Polyklonaler Antikörper

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• KD/KO Validated

MGAT4C Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17841-1-AP

Synonyme

GlcNAc T IVc, GnT IVc, GNTIVH, HGNT IV H, MGAT4C

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  WB analysis using 17841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17841-1-AP (MGAT4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17841-1-AP

  IP result of anti-MGAT4C (IP:17841-1-AP, 4ug; Detection:17841-1-AP 1:400) with mouse testis tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

17841-1-AP bindet in WB, IP, IHC, ELISA MGAT4C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MGAT4C fusion protein Ag12201
• Vollständiger Name: mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme C (putative)
• Berechnetes Molekulargewicht: 478 aa, 56 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC064141
• Gene symbol: MGAT4C
• Gene ID (NCBI): 25834
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MGAT4C is predicted to be a glycosyltransferase that catalyzes a GlcNAc β1-4 linkage to the core mannose residues of N-glycans, resulting in N-glycans with tri- or tetraantennary structures.Recently MGAT4C has been found to get involved in processing complex N-glycan structures on CD133 and CD133 has been identified as a substrate of MGAT4C. Given to the fact that CD133 is widely used as a marker of numerous stem cell and cancer stem cell types, MGAT4C may have an important role in certain cancers. (PMID: 21937449)

Publikationen

Species	Application	Title
mouse	IHC	Dev Cell; Wound-healing plasticity enables clonal expansion of founder progenitor cells in colitis; Authors - Cambrian Y Liu
human	WB	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB	J Biol Chem; CD133 protein N-glycosylation processing contributes to cell surface recognition of the primitive cell marker AC133 epitope.; Authors - Mak Anthony B AB ; KD Validated