MGC33407/ACTL9 Polyklonaler Antikörper

MGC33407/ACTL9 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29885-1-PBS

Synonyme

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  WB analysis using 29885-1-AP (same clone as 29885-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 29885-1-AP (same clone as 29885-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 29885-1-AP (same clone as 29885-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29885-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA MGC33407/ACTL9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MGC33407/ACTL9 fusion protein Ag31805
• Vollständiger Name: hypothetical protein MGC33407
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC024028
• Gene symbol: ACTL9
• Gene ID (NCBI): 284382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACTL9 (Actin Like 9), also named MGC33407, encodes a testis-specific actin-like protein that plays a role in fusion of proacrosomal vesicles and perinuclear theca formation (PMID: 33626338). ACTL9 protein contains 416 amino acids, which mainly localizes in the equatorial segment of human sperm head (PubMed: 33626338). Diseases associated with ACTL9 include spermatogenic failure and non-syndromic male infertility.