MGEA5 Monoklonaler Antikörper

MGEA5 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

3B9H7

Kat-Nr. : 66033-1-Ig

Synonyme

OGA, 3B9H7, Beta-hexosaminidase, Beta-N-acetylglucosaminidase, Beta-N-acetylhexosaminidase

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  WB analysis of Human brain using 66033-1-Ig

  Human brain lysate were subjected to SDS PAGE followed by western blot with 66033-1-Ig (MGEA5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 66033-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 66033-1-Ig (MGEA5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 66033-1-Ig(MGEA5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human meningioma using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 66033-1-Ig (MGEA5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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  IHC staining of human meningioma using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 66033-1-Ig (MGEA5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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  IHC staining of human brain using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66033-1-Ig(MGEA5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66033-1-Ig(MGEA5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66033-1-Ig (MGEA5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human gliomas using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66033-1-Ig (MGEA5 Antibody) at dilution of 1:200 (under 40x lens).Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human gliomas using 66033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 66033-1-Ig(MGEA5 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 66033-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66033-1-Ig (MGEA5 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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  IF Staining of HeLa using 66033-1-Ig

  Immunofluorescent analysis of Hela cells, using MGEA5 antibody 66033-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, MCF7 cells
• Erfolgreiche Detektion in IHC: humanes Meningiom-Gewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66033-1-Ig bindet in WB, IHC, IF/ICC, ELISA MGEA5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MGEA5 fusion protein Ag6905
• Vollständiger Name: meningioma expressed antigen 5 (hyaluronidase)
• Berechnetes Molekulargewicht: 103 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 130 kDa
• GenBank-Zugangsnummer: BC039583
• Gene symbol: MGEA5
• Gene ID (NCBI): 10724
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present mouse monopolyclonal antibody 66033-1-Ig, which is consistent with results in a related reference (PubMed:11341771).

Publikationen

Species	Application	Title
human	WB	Cell Metab; Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fraction; Authors - Yuki Tatekoshi
mouse	WB	Res Sq; Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction; Authors - Hossein Ardehali