Tested Applications
| Positive WB detected in | SMMC-7721 cells, BxPC-3 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse pancreas tissue, human meningioma tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14711-1-AP targets MGEA5 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, zebrafish, drosophila |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6405 Product name: Recombinant human MGEA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC039583 Sequence: MVQKESQATLEERESELSSNPAASAGASLEPPAAPAPGEDNPAGAGGAAVAGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMSRQVA Predict reactive species |
| Full Name | meningioma expressed antigen 5 (hyaluronidase) |
| Calculated Molecular Weight | 103 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC039583 |
| Gene Symbol | MGEA5 |
| Gene ID (NCBI) | 10724 |
| RRID | AB_2143063 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60502 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present rabbit polyclonal antibody 14711-1-AP, which is consistent with results in a related reference (PubMed:11341771).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MGEA5 antibody 14711-1-AP | Download protocol |
| IF protocol for MGEA5 antibody 14711-1-AP | Download protocol |
| IHC protocol for MGEA5 antibody 14711-1-AP | Download protocol |
| IP protocol for MGEA5 antibody 14711-1-AP | Download protocol |
| WB protocol for MGEA5 antibody 14711-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-O-GlcNAc antibody (Cat# 14711-1-AP) has accumulated 111 citations across journals including Nature Communications, Nature Cell Biology, Molecular Cell, JBC, Cell Reports, EMBO Journal, and PNAS. Research fields span O-GlcNAcylation/glycosylation biology, neuroscience (Alzheimer's, Parkinson's), cardiology, oncology, metabolism (diabetes, obesity), immunology, developmental biology, and renal disease.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins | ||
Nat Commun Renal tubular GSDME protects cisplatin nephrotoxicity by impeding OGT-STAT3-S100A7A axis in male mice | ||
Nat Commun Fibroblast-specific PRMT5 deficiency suppresses cardiac fibrosis and left ventricular dysfunction in male mice | ||
Nat Commun YBX1 Confers immunosuppressive bone metastatic traits in non-small cell lung cancer. | ||
J Adv Res O-GlcNAc modification mediates aquaporin 3 to coordinate endometrial cell glycolysis and affects embryo implantation. |
Reviews
What customers say
The single available review (Feb 2019) rates the product 2 out of 5 stars. The reviewer used it for Western Blot on C2C12 cells at a 1:1000 dilution and noted that the antibody consistently produced two bands together, suggesting potential specificity or resolution concerns in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wei (Verified Customer) (02-28-2019) | Always two bands together
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