MGEA5 Rekombinanter Antikörper

MGEA5 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242263A6

Kat-Nr. : 84722-4-PBS

Synonyme

OGA, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase, Beta-N-acetylglucosaminidase

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  WB analysis using 84722-4-RR (same clone as 84722-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84722-4-RR (MGEA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84722-4-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 84722-4-RR (same clone as 84722-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84722-4-RR (MGEA5 antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84722-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84722-4-RR (same clone as 84722-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGEA5 antibody (84722-4-RR, Clone: 242263A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84722-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84722-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84722-4-RR against Human MGEA5 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84722-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MGEA5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MGEA5 fusion protein Ag6405
• Vollständiger Name: meningioma expressed antigen 5 (hyaluronidase)
• Berechnetes Molekulargewicht: 103 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: BC039583
• Gene symbol: MGEA5
• Gene ID (NCBI): 10724
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present rabbit polyclonal antibody 14711-1-AP, which is consistent with results in a related reference (PubMed:11341771).