MGLL Polyklonaler Antikörper

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• KD/KO Validated

MGLL Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14986-1-PBS

Synonyme

EC:3.1.1.23, HU K5, HUK5, HU-K5, Monoacylglycerol lipase

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  WB analysis of HepG2 using 14986-1-AP (same clone as 14986-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14986-1-AP (MGLL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

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  WB analysis using 14986-1-AP (same clone as 14986-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14986-1-AP (MGLL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 14986-1-AP (same clone as 14986-1-PBS)

  IP result of anti-MGLL (IP:14986-1-AP, 4ug; Detection:14986-1-AP 1:300) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14986-1-PBS

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14986-1-PBS

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14986-1-AP (same clone as 14986-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 14986-1-AP (same clone as 14986-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14986-1-PBS bindet in WB, IHC, IP, ELISA MGLL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MGLL fusion protein Ag6970
• Vollständiger Name: monoglyceride lipase
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC000551
• Gene symbol: MGLL
• Gene ID (NCBI): 11343
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MGLL (monoglyceride lipase) is also named as MAGL, HU-K5 and belongs to the monoacylglycerol lipase family. It converts monoacylglycerides to free fatty acids and glycerol and functions together with hormone-sensitive lipase (HSL) in the mobilization of fatty acids from the triglyceride stores of adipocytes. MGL protein is ubiquitously expressed in kidney, spleen, heart, liver, stomach, brain, lung, adrenal gland, adipose tissue and in brain,there are two bands of 33 kDa and 35 kDa can be detected. The single protein species expressed in muscle is larger (around 40 kDa) (PMID:11470505). This protein can exsit as a homodimer (PMID:19957260).