MGME1 Monoklonaler Antikörper

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• KD/KO Validated

MGME1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2C4G1

Kat-Nr. : 67468-1-Ig

Synonyme

C20orf72, 2C4G1, DDK1, EC:3.1.-.-, Mitochondrial genome maintenance exonuclease 1

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  WB analysis using 67468-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67468-1-Ig (MGME1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67468-1-Ig

  WB result of MGME1 antibody (67468-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGME1 transfected HeLa cells.

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  WB analysis of HL-60 using 67468-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67468-1-Ig (MGME1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IP: See 1 publications below

Produktinformation

67468-1-Ig bindet in WB, IHC, IP, ELISA MGME1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MGME1 fusion protein Ag19644
• Vollständiger Name: chromosome 20 open reading frame 72
• Berechnetes Molekulargewicht: 344 aa, 39 kDa
• Beobachtetes Molekulargewicht: 36-39 kDa
• GenBank-Zugangsnummer: BC016869
• Gene symbol: MGME1
• Gene ID (NCBI): 92667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Aging (Albany NY); MGME1 associates with poor prognosis and is vital for cell proliferation in lower-grade glioma; Authors - Feng Xiao
human	WB,IP	Biochem Biophys Res Commun; Unspliced XBP1 enhences metabolic reprogramming in colorectal cancer cells by interfering with the mitochondrial localization of MGME1; Authors - Jiandong Zhang