MGMT Polyklonaler Antikörper

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• KD/KO Validated

MGMT Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Kat-Nr. : 17195-1-AP

Synonyme

O-6-methylguanine-DNA-alkyltransferase, Methylated-DNA--protein-cysteine methyltransferase, EC:2.1.1.63, 6-O-methylguanine-DNA methyltransferase

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  WB analysis using 17195-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17195-1-AP (MGMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17195-1-AP

  WB result of MGMT antibody (17195-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGMT transfected HeLa cells.

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  WB analysis of Jurkat using 17195-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17195-1-AP (MGMT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17195-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17195-1-AP (MGMT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 17195-1-AP

  IP result of anti-MGMT (IP:17195-1-AP, 4ug; Detection:17195-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of human liver cancer using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17195-1-AP (MGMT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17195-1-AP (MGMT antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17195-1-AP (MGMT antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17195-1-AP (MGMT antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17195-1-AP (MGMT antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17195-1-AP (MGMT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17195-1-AP (MGMT antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17195-1-AP (MGMT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 17195-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human MGMT (17195-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Plazenta-Gewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

17195-1-AP bindet in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA MGMT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MGMT fusion protein Ag9996
• Vollständiger Name: O-6-methylguanine-DNA methyltransferase
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC000824
• Gene symbol: MGMT
• Gene ID (NCBI): 4255
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment [PMID:23065697].MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine [PMID: 17482892]. The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea.[PMID: 10767620]

Publikationen

Species	Application	Title
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	Nucleic Acids Res; KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response.; Authors - Mingming Yang
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
human,mouse	WB,IHC	J Pharm Anal; MGMT activated by Wnt pathway promotes cisplatin tolerance through inducing slow-cycling cells and nonhomologous end joining in colorectal cancer; Authors - Haowei Zhang ; KD Validated
human	WB	Oncogene; GBP3 promotes glioblastoma resistance to temozolomide by enhancing DNA damage repair.; Authors - Hui Xu
human	IHC	Oncogenesis; GSK3β palmitoylation mediated by ZDHHC4 promotes tumorigenicity of glioblastoma stem cells in temozolomide-resistant glioblastoma through the EZH2-STAT3 axis.; Authors - Chenggang Zhao