MGMT Rekombinanter Antikörper

MGMT Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

243130D8

Kat-Nr. : 86115-2-PBS

Synonyme

6-O-methylguanine-DNA methyltransferase, EC:2.1.1.63, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase

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  Sandwich ELISA standard curve of MP02085-2

  Sandwich ELISA standard curve of MP02085-2, Human MGMT Recombinant Matched Antibody Pair - PBS only. 89933-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9996. 89933-6-PBS was HRP conjugated as the detection antibody. Range: 0.781-25 ng/mL.

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  Sample test of MP02085-2

  The mean MGMT concentration was determined to be 32.91 ng/mL in Jurkat cell extract based on a 1.90 mg/mL extract load and 17.53 ng/mL in SH-SY5Y cell extract based on a 1.90 mg/mL extract load.

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  Affinity and Kinetic Characterization of 86115-2-PBS

  Biolayer interferometry (BLl) kinetic assays of 86115-2-PBS against Human MGMT were performed. The affinity constant is 0.659 nM.

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Geprüfte Anwendungen

Produktinformation

86115-2-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test MGMT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MGMT fusion protein Ag9996
• Vollständiger Name: O-6-methylguanine-DNA methyltransferase
• Berechnetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC000824
• Gene symbol: MGMT
• Gene ID (NCBI): 4255
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.