MGMT Rekombinanter Antikörper

MGMT Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243130G10

Kat-Nr. : 86115-5-PBS

Synonyme

6-O-methylguanine-DNA methyltransferase, EC:2.1.1.63, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase

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  WB analysis using 86115-5-RR (same clone as 86115-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 89933-4-RR (MGMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 86115-5-RR (same clone as 86115-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86115-5-RR (MGMT antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 86115-5-RR (same clone as 86115-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86115-5-RR (MGMT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 86115-5-RR (same clone as 86115-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MGMT antibody (89933-4-RR, Clone: 243130G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86115-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MGMT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MGMT fusion protein Ag9996
• Vollständiger Name: O-6-methylguanine-DNA methyltransferase
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC000824
• Gene symbol: MGMT
• Gene ID (NCBI): 4255
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment (PMID:23065697). MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine (PMID: 17482892). The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea (PMID: 10767620).