MGP Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

MGP Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

1A1C3

Kat-Nr. : 60055-1-Ig

Synonyme

GIG36, 1A1C3, Cell growth-inhibiting gene 36 protein, matrix Gla protein, MGLAP

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  WB analysis using 60055-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 60055-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 60055-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 60055-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver cancer using 60055-1-Ig

  human liver cancer tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60055-1-Ig (MGP antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human gliomas using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens).

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  IHC staining of human heart using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human heart using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens).

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  IHC staining of human kidney using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens).

• 

  IHC staining of human kidney using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens).

• 

  IHC staining of human brain using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse kidney using 60055-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MGP antibody (60055-1-Ig, Clone: 1A1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  Affinity and Kinetic Characterization of 60055-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60055-1-Ig against Human MGP were performed. The Affinity Constant is 5.42 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, humanes Hirngewebe, humanes Nierengewebe, humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Hirngewebe, humanes Kolonkarzinomgewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

60055-1-Ig bindet in WB, IHC, IF-P, ELISA MGP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MGP fusion protein Ag1091
• Vollständiger Name: matrix Gla protein
• Berechnetes Molekulargewicht: 103 aa, 13 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC005272
• Gene symbol: MGP
• Gene ID (NCBI): 4256
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Matrix Gla protein (MGP) is is a vitamin K-dependent, extracellular matrix protein. MGP plays a pivotal role in preventing soft tissue calcification and local mineralization of the vascular wall. Vitamin K deficiency leads to inactive uncarboxylated MGP (ucMGP), which accumulates at sites of arterial calcification. However MGP is synthesized in many tissues and is especially enriched in embryonic tissues and in cancer cells. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.

Publikationen

Species	Application	Title
human	WB	J Inflamm Res; circ_TGFBR2 Inhibits Vascular Smooth Muscle Cells Phenotypic Switch and Suppresses Aortic Dissection Progression by Sponging miR-29a; Authors - Zhenjun Xu
human	WB	J Biol Chem; Reduced APPL1 impairs osteogenic differentiation of mesenchymal stem cells by facilitating MGP expression to disrupt the BMP2 pathway in osteoporosis; Authors - Weiquan Yuan ; KD Validated
human	WB,IF	Int Immunopharmacol; MGP+ and IDO1+ tumor-associated macrophages facilitate immunoresistance in breast cancer revealed by single-cell RNA sequencing; Authors - Kexin Chang
mouse	IHC	Adv Sci (Weinh); Mgp High-Expressing MSCs Orchestrate the Osteoimmune Microenvironment of Collagen/Nanohydroxyapatite-Mediated Bone Regeneration; Authors - Zhuqing Wan
	IF	Nat Commun; Decoding the mechanical characteristics of the human anterior cruciate ligament entheses through graduated mineralization interfaces; Authors - Jinghua Fang
mouse	IHC	Mol Ther; Dysregulation of Astrocyte-derived Matrix Gla Protein Impairs Dendritic Spine Development in Pyridoxine-dependent Epilepsy; Authors - Junjie Wu