MGP Monoklonaler Antikörper

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MGP Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A1C3

Kat-Nr. : 60055-1-PBS

Synonyme

GIG36, 1A1C3, Cell growth-inhibiting gene 36 protein, matrix Gla protein, MGLAP

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  WB analysis using 60055-1-Ig (same clone as 60055-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 60055-1-Ig (same clone as 60055-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 60055-1-Ig (same clone as 60055-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

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  WB analysis of human saliva using 60055-1-Ig (same clone as 60055-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver cancer using 60055-1-Ig (same clone as 60055-1-PBS)

  human liver cancer tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60055-1-Ig (MGP antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 60055-1-Ig (same clone as 60055-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MGP antibody (60055-1-Ig, Clone: 1A1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 60055-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60055-1-Ig against Human MGP were performed. The Affinity Constant is 5.42 nM.

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Geprüfte Anwendungen

Produktinformation

60055-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA MGP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MGP fusion protein Ag1091
• Vollständiger Name: matrix Gla protein
• Berechnetes Molekulargewicht: 103 aa, 13 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC005272
• Gene symbol: MGP
• Gene ID (NCBI): 4256
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Matrix Gla protein (MGP) is is a vitamin K-dependent, extracellular matrix protein. MGP plays a pivotal role in preventing soft tissue calcification and local mineralization of the vascular wall. Vitamin K deficiency leads to inactive uncarboxylated MGP (ucMGP), which accumulates at sites of arterial calcification. However MGP is synthesized in many tissues and is especially enriched in embryonic tissues and in cancer cells. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.