RNF156 Polyklonaler Antikörper

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• KD/KO Validated

RNF156 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 11285-1-AP

Synonyme

KIAA0544, mahogunin, ring finger 1, MGRN1, RING finger protein 156, RNF156

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  WB analysis using 11285-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11285-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11285-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11285-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11285-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11285-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11285-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11285-1-AP (RNF156 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11285-1-AP (RNF156 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11285-1-AP (RNF156 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11285-1-AP (RNF156 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

11285-1-AP bindet in WB, IHC, IF, IP, ELISA RNF156 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF156 fusion protein Ag1808
• Vollständiger Name: mahogunin, ring finger 1
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 58-64 kDa
• GenBank-Zugangsnummer: BC014199
• Gene symbol: MGRN1
• Gene ID (NCBI): 23295
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MGRN1(E3 ubiquitin-protein ligase) mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. It also plays a role in the regulation of endosome-to-lysosome trafficking. Human and mouse mahogunin exhibit a similar expression pattern across multiple tissues with particularly high levels in brain(PMID: 12560552). It has 4 isoforms produced by alternative splicing with the molecular mass of 58-64 kDa.

Publikationen

Species	Application	Title
human	WB,IF	Mol Cell; Aging Triggers Cytoplasmic Depletion and Nuclear Translocation of the E3 Ligase Mahogunin: A Function for Ubiquitin in Neuronal Survival.; Authors - Stefano Benvegnù ; KD Validated
human	WB	Dev Cell; A Membrane-Tethered Ubiquitination Pathway Regulates Hedgehog Signaling and Heart Development.; Authors - Jennifer H Kong
human	WB	Pigment Cell Melanoma Res; MGRN1-dependent pigment-type switching requires its ubiquitination activity but not its interaction with TSG101 or NEDD4.; Authors - Gunn Teresa M TM
human,mouse	IP	Development; Gene-teratogen interactions influence the penetrance of birth defects by altering Hedgehog signaling strength.; Authors - Jennifer H Kong
human	WB,IHC	Front Oncol; Promoter Methylation of the MGRN1 Gene Predicts Prognosis and Response to Chemotherapy of High-Grade Serous Ovarian Cancer Patients.; Authors - Xiao-Fei Li
mouse	WB,IF	J Biol Chem; Mahoganoid and mahogany mutations rectify the obesity of the yellow mouse by effects on endosomal traffic of MC4R protein.; Authors - Overton John D JD ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parijat (Verified Customer) (09-20-2023)

Works well.

• Applications: Western Blot