MHC Class II (I-A/I-E) Rekombinanter Antikörper

MHC Class II (I-A/I-E) Rekombinant Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

M5/114.15.2

Kat-Nr. : 65663-1-TR

Synonyme

H2Aa, Aalpha, H 2Aa, H2-Aa, Ia 1

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  FC experiment of mouse splenocytes using 65663-1-TR

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rat IgG2a RecAb (65663-1-TR, Clone: M5/114.15.2) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11) (CL647-65060, Clone: 145-2C11). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 65663-1-TR

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rat IgG2a RecAb (65663-1-TR, Clone: M5/114.15.2) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65663-1-TR bindet in FC MHC Class II (I-A/I-E) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: histocompatibility 2, class II antigen A, alpha
• GenBank-Zugangsnummer: BC031711
• Gene symbol: MHC Class II
• Gene ID (NCBI): 14960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 antibody is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).