MHC Class II (I-A/I-E) Rekombinanter Antikörper

MHC Class II (I-A/I-E) Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE-Cyanine7 Fluorescent Dye

CloneNo.

M5/114.15.2

Kat-Nr. : PY7-FcA65663

Synonyme

H2Aa, Aalpha, H 2Aa, H2-Aa, Ia 1

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  FC experiment of mouse splenocytes using PY7-FcA65663

  1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ CoraLite® Plus 488 Anti-Mouse CD3 (17A2), and 0.25 ug PE-Cyanine7 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rabbit IgG RecAb (PY7-FcA65663, Clone: M5/114.15.2) or FcZero-rAb™ PE-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (PY7-FcA98136, Clone: 240953C9). Cells were incubated with MonoZero™ Monocytes blocking Reagent (PF00020) prior to staining. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PY7-FcA65663

  1x10^6 mouse splenocytes were surface stained with 0.25 ug PE-Cyanine7 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rabbit IgG RecAb (PY7-FcA65663, Clone: M5/114.15.2) (red) or FcZero-rAb™ PE-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (PY7-FcA98136, Clone: 240953C9) (blue). Cells were incubated with MonoZero™ Monocytes blocking Reagent (PF00020) prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PY7-FcA65663 bindet in FC MHC Class II (I-A/I-E) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: histocompatibility 2, class II antigen A, alpha
• GenBank-Zugangsnummer: BC031711
• Gene symbol: MHC Class II
• Gene ID (NCBI): 14960
• Konjugation: PE-Cyanine7 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 450-500 nm / 778 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 antibody is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).