MIA3 Polyklonaler Antikörper

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• KD/KO Validated

MIA3 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 17481-1-AP

Synonyme

ARNT, C219 reactive peptide, D320, KIAA0268, MIA3, TANGO, TANGO1, UNQ6077

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  WB analysis of HEK-293T using 17481-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 17481-1-AP (MIA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17481-1-AP

  IP result of anti-MIA3 (IP:17481-1-AP, 5ug; Detection:17481-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of mouse testis using 17481-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 17481-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17481-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17481-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

17481-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA MIA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIA3 fusion protein Ag11514
• Vollständiger Name: melanoma inhibitory activity family, member 3
• Berechnetes Molekulargewicht: 214 kDa
• Beobachtetes Molekulargewicht: 300 kDa
• GenBank-Zugangsnummer: BC047116
• Gene symbol: MIA3
• Gene ID (NCBI): 375056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Melanoma inhibitory activity member 3 (MIA3/TANGO1) is an evolutionarily conserved endoplasmic reticulum resident transmembrane protein.

Publikationen

Species	Application	Title
human	WB,IF	Front Endocrinol (Lausanne); MIA SH3 Domain ER Export Factor 3 Deficiency Prevents Neointimal Formation by Restoring BAT-Like PVAT and Decreasing VSMC Proliferation and Migration.; Authors - Yu Lei ; KD Validated
human	WB,IF	Arthritis Rheumatol; Caspase-1 Enhances TANGO1 Expression to Promote Procollagen Export from the Endoplasmic Reticulum in Systemic Sclerosis Contributing to Fibrosis; Authors - Lianne M Connolly ; KD Validated
zebrafish	IF	Elife; Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset; Authors - Arian Mansur
human	WB,IHC,IF,CoIP	Mol Cell Biochem; MIA3 promotes the degradation of GSH (glutathione) by binding to CHAC1, thereby promoting the progression of hepatocellular carcinoma; Authors - Zhou Wanbiao ; KD Validated
		Dev Cell; Endoplasmic reticulum exit sites are segregated for secretion based on cargo size; Authors - Sonashree Saxena