MIA3 Polyklonaler Antikörper

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MIA3 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17481-1-PBS

Synonyme

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  WB analysis of HEK-293T using 17481-1-AP (same clone as 17481-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 17481-1-AP (MIA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 17481-1-AP (same clone as 17481-1-PBS)

  IP result of anti-MIA3 (IP:17481-1-AP, 5ug; Detection:17481-1-AP 1:1000) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 17481-1-AP (same clone as 17481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 17481-1-AP (same clone as 17481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17481-1-AP (same clone as 17481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 17481-1-AP (same clone as 17481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17481-1-AP (MIA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17481-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17481-1-PBS bindet in WB, IHC, IP, Indirect ELISA MIA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIA3 fusion protein Ag11514
• Vollständiger Name: melanoma inhibitory activity family, member 3
• Berechnetes Molekulargewicht: 214 kDa
• Beobachtetes Molekulargewicht: 300 kDa
• GenBank-Zugangsnummer: BC047116
• Gene symbol: MIA3
• Gene ID (NCBI): 375056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Melanoma inhibitory activity member 3 (MIA3/TANGO1) is an evolutionarily conserved endoplasmic reticulum resident transmembrane protein.