MIB1 Polyklonaler Antikörper

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• KD/KO Validated

MIB1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 11893-1-AP

Synonyme

DAPK interacting protein 1, DIP 1, DIP1, KIAA1323, MIB, MIB1, Mind bomb homolog 1, ZZANK2, ZZZ6

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Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 11893-1-AP

  WB result of MIB1 antibody (11893-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MIB1 transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 11893-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11893-1-AP (MIB1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 11893-1-AP

  IP Result of anti-MIB1 (IP:11893-1-AP, 4ug; Detection:11893-1-AP 1:500) with K-562 cells lysate 3400ug.

• 

  IHC staining of human kidney using 11893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11893-1-AP (MIB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11893-1-AP (MIB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

11893-1-AP bindet in WB, IP, IHC, ELISA MIB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIB1 fusion protein Ag2507
• Vollständiger Name: mindbomb homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 110 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC022403
• Gene symbol: MIB1
• Gene ID (NCBI): 57534
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MIB1(Mind bomb homolog 1) is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. MIB1, identified as an interactor of death associated protein kinase (DAPK), probably regulate apoptosis through ubiquitination and degradation of DAPK, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. MIB1 promotes proteasomal degradation of NRF2 and sensitizes lung cancer cells to ferroptosis(PMID: 34670864). In addition, MIB1 upregulates IQGAP1 and promotes pancreatic cancer progression by inducing ST7 degradation(PMID: 33793053).

Publikationen

Species	Application	Title
human	WB	Cancer Res; Cancer-Specific Targeting of Taurine-Upregulated Gene 1 Enhances the Effects of Chemotherapy in Pancreatic Cancer.; Authors - Yoshihiko Tasaki
mouse	WB	Mol Med; Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis; Authors - Juan Xiao ; KD Validated
human	WB	Cell Death Dis; hPER3 promotes adipogenesis via hHSP90AA1-mediated inhibition of Notch1 pathway.; Authors - Xinxing Wan
human	WB	MedComm (2020); Sanggenon C inhibits cell proliferation and induces apoptosis by regulating the MIB1/DAPK1 axis in glioblastoma; Authors - Hongbo Chang
human,mouse	WB,IHC	J Cell Mol Med; SNORA73B promotes endometrial cancer progression through targeting MIB1 and regulating host gene RCC1 alternative splicing; Authors - Xi Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xin (Verified Customer) (04-22-2021)

Good antibody, clear band seen in parental vs MIB1 knockout A549 cell

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549

MIB1 Antibody Western Blot validation (1:1000 dilution) in A549 (Cat no:11893-1-AP)