MICA Rekombinanter Antikörper

MICA Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240735D8

Kat-Nr. : 83725-3-PBS

Synonyme

MIC A, MIC-A

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  Sandwich ELISA standard curve of MP00686-1

  Sandwich ELISA standard curve of MP00686-1, Human MICA Recombinant Matched Antibody Pair - PBS only. 83725-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1176. 83725-1-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 pg/mL.

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  Sample test of MP00686-1

  HUVEC cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MICA, and measured 70.4 pg/mL.
  HeLa cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MICA, and measured 285.9 pg/mL.

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  Cytometric bead array standard curve of MP00686-1

  Cytometric bead array standard curve of MP00686-1, MICA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83725-3-PBS. Detection antibody: 83725-1-PBS. Standard: Eg1176. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83725-3-PBS

  Biolayer interferometry (BLl) kinetic assays of 83725-3-PBS against Human MICA were performed. The affinity constant is 0.819 nM.

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Geprüfte Anwendungen

Produktinformation

83725-3-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test MICA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MICA fusion protein Eg1176
• Vollständiger Name: MHC class I polypeptide-related sequence A
• Berechnetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: AAH16929.1
• Gene symbol: MICA
• Gene ID (NCBI): 100507436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.