• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MICA/MICB Rekombinanter Antikörper

MICA/MICB Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240735D9

Kat-Nr. : 83725-5-PBS

Synonyme

MICA, PERB11.1, MIC A, 240735D9

Filter:
  • Western Blot (WB) analysis of various lysates using MICA/MICB Recombinant antibody (83725-5-RR)

    WB analysis using 83725-5-RR (same clone as 83725-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83725-5-RR (MICA/MICB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83725-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83725-5-RR

    Affinity and Kinetic Characterization of 83725-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83725-5-RR against Human MICA were performed. The affinity constant is 1.55 nM.

Geprüfte Anwendungen

Produktinformation

83725-5-PBS bindet in WB, ELISA MICA/MICB und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name MHC class I polypeptide-related sequence A
Berechnetes Molekulargewicht43 kDa
Beobachtetes Molekulargewicht50-60 kDa
GenBank-ZugangsnummerAAH16929.1
Gene symbol MICA
Gene ID (NCBI) 100507436
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). This antibody recognizes both MICA and MICB.