MICA/MICB Rekombinanter Antikörper

MICA/MICB Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

6D4

Kat-Nr. : 65589-1-MR

Synonyme

MICB, MICA, 6D4, MIC A, MIC-A

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  FC experiment of HeLa using 65589-1-MR

  1x10^6 HeLa cells were surface stained with 0.2 ug Anti-Human MICA/MICB Mouse IgG2a Recombinant Antibody (65589-1-MR, Clone: 6D4) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65589-1-MR bindet in FC MICA/MICB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Transfizierte MICA-exprimierende C1R-Zellen
• Vollständiger Name: MHC class I polypeptide-related sequence A
• Berechnetes Molekulargewicht: 383 aa, 43 kDa
• GenBank-Zugangsnummer: BC016929
• Gene symbol: MICA
• Gene ID (NCBI): 100507436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.