MICA/MICB Rekombinanter Antikörper

MICA/MICB Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

6D4

Kat-Nr. : 65589-1-PBS

Synonyme

MICB, MICA, 6D4, MIC A, MIC-A

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  FC experiment of HeLa using 65589-1-MR (same clone as 65589-1-PBS)

  1x10^6 HeLa cells were surface stained with 0.2 ug Anti-Human MICA/MICB Mouse IgG2a Recombinant Antibody (65589-1-MR, Clone: 6D4) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 65589-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65589-1-PBS bindet in FC MICA/MICB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Transfizierte MICA-exprimierende C1R-Zellen
• Vollständiger Name: MHC class I polypeptide-related sequence A
• Berechnetes Molekulargewicht: 383 aa, 43 kDa
• GenBank-Zugangsnummer: BC016929
• Gene symbol: MICA
• Gene ID (NCBI): 100507436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.