MICA/MICB Monoklonaler Antikörper

MICA/MICB Monoklonal Antikörper für IHC, FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

human

Anwendung

IHC, FC

Konjugation

Biotin

CloneNo.

6D4

Kat-Nr. : Biotin-65161

Synonyme

MICA, MIC-A, MIC A, 6D4

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  IHC staining of human colon using Biotin-65161

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using Biotin-65161

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using Biotin-65161

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using Biotin-65161

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using Biotin-65161

  1x10^6 HeLa cells were surface stained with 0.5 ug Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161, Clone:6D4) or isotype control, and SA-PE. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-65161 bindet in IHC, FC MICA/MICB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Transfizierte MICA-exprimierende C1R-Zellen
• Vollständiger Name: MHC class I polypeptide-related sequence A
• Berechnetes Molekulargewicht: 383 aa, 43 kDa
• GenBank-Zugangsnummer: BC016929
• Gene symbol: MICA
• Gene ID (NCBI): 100507436
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit Biotin-LC-NHS unter optimalen Bedingungen konjugiert. Das Reagenz ist frei von unkonjugiertem Biotin.
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.