MICAL1 Monoklonaler Antikörper

MICAL1 Monoklonal Antikörper für WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3F5D4

Kat-Nr. : 68297-1-PBS

Synonyme

NICAL, NEDD9-interacting protein with calponin homology and LIM domains, MICAL, EC:1.14.13.225, 3F5D4

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  WB analysis using 68297-1-Ig (same clone as 68297-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68297-1-Ig (MICAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68297-1-Ig (same clone as 68297-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68297-1-Ig (MICAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 68297-1-Ig (same clone as 68297-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68297-1-Ig (MICAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68297-1-Ig (same clone as 68297-1-PBS)

  IP result of anti-MICAL1 (IP:68297-1-Ig, 4ug; Detection:68297-1-Ig 1:2000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68297-1-Ig (same clone as 68297-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MICAL1 antibody (68297-1-Ig, Clone: 3F5D4 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68297-1-Ig (same clone as 68297-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MICAL1 (68297-1-Ig, Clone:3F5D4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68297-1-PBS bindet in WB, IF/ICC, FC (Intra), IP, Indirect ELISA MICAL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MICAL1 fusion protein Ag6578
• Vollständiger Name: microtubule associated monoxygenase, calponin and LIM domain containing 1
• Berechnetes Molekulargewicht: 118 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC052983
• Gene symbol: MICAL1
• Gene ID (NCBI): 64780
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.