MICAL2 Polyklonaler Antikörper

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• KD/KO Validated

MICAL2 Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-13965

Synonyme

DKFZp686H03148, DKFZp686H2469, KIAA0750, MICAL2, MICAL2PV1, MICAL2PV2, Protein MICAL 2

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL488-13965

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 MICAL2 antibody (CL488-13965) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-13965 bindet in IF MICAL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MICAL2 fusion protein Ag4950
• Vollständiger Name: microtubule associated monoxygenase, calponin and LIM domain containing 2
• Berechnetes Molekulargewicht: 127 kDa
• Beobachtetes Molekulargewicht: 95 kDa, 112 kDa
• GenBank-Zugangsnummer: BC044577
• Gene symbol: MICAL2
• Gene ID (NCBI): 9645
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL2 has three conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain and a Lin11, Isl-1 and Mec-3 (LIM) domain. It has been demonstrated that MICAL2 could regulate actin stress fibers and is required for normal actin organization. In addition, MICAL2-PV, a novel splicing variants of MICAL2, has been reported to be involved in cancer progression of prostate cancer. This antibody can recognize both MICAL2 and MICAL2-PV. This antibody recognizes various isoforms of MICAL2 around 90-95 kDa or 109-112 kDa.