MICALL2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MICALL2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (1)

Kat-Nr. : 24408-1-AP

Synonyme

JRAB, MICAL L2, MICAL like 2, MICAL like protein 2, MICALL2

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• 

  WB analysis of PC-3 using 24408-1-AP

  WB result of MICALL2 antibody (24408-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MICALL2 transfected PC-3 cells.

• 

  WB analysis using 24408-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24408-1-AP (MICALL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 24408-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24408-1-AP (MICALL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HeLa-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

24408-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA MICALL2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MICALL2 fusion protein Ag19931
• Vollständiger Name: MICAL-like 2
• Berechnetes Molekulargewicht: 904 aa, 98 kDa
• Beobachtetes Molekulargewicht: 109 kDa
• GenBank-Zugangsnummer: BC037988
• Gene symbol: MICALL2
• Gene ID (NCBI): 79778
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Int J Mol Sci; Comprehensive Analysis of MICALL2 Reveals Its Potential Roles in EGFR Stabilization and Ovarian Cancer Cell Invasion; Authors - Tianxiang Xia ; KD Validated
human	WB,IHC,IF	Biochem Pharmacol; MICAL-L2, as an estrogen-responsive gene, is involved in ER-positive breast cancer cell progression and tamoxifen sensitivity via the AKT/mTOR pathway; Authors - Pushuai Wen ; KD Validated
human	WB,IHC,IF,CoIP	Biochim Biophys Acta Mol Basis Dis; High MICAL-L2 promotes cancer progression and drug resistance in renal clear cell carcinoma cells through stabilization of ACTN4 following vimentin expression; Authors - Weizhen Zhao ; KD Validated
human	WB,IHC,IF,IP	Cell Commun Signal; MICALL2 as a substrate of ubiquitinase TRIM21 regulates tumorigenesis of colorectal cancer; Authors - Pushuai Wen ; KD Validated
mouse	IF	Arch Biochem Biophys; MICALL2 participates in the regulation of epithelial-mesenchymal transition in alveolar epithelial cells - Potential roles in pulmonary fibrosis; Authors - Yingying Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (05-04-2019)

WB required long exposure >100s. Multiple background bands. Molecular weight is BioRad Precision Plus.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

MICALL2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:24408-1-AP)