MIDN Polyklonaler Antikörper

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• KD/KO Validated

MIDN Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 18939-1-AP

Synonyme

Midnolin

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  WB analysis using 18939-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18939-1-AP (MIDN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 18939-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18939-1-AP (MIDN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 18939-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18939-1-AP (MIDN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, BxPC-3-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

18939-1-AP bindet in WB, IHC, IF, ELISA MIDN und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIDN fusion protein Ag13492
• Vollständiger Name: midnolin
• Berechnetes Molekulargewicht: 468 aa, 49 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC094778
• Gene symbol: MIDN
• Gene ID (NCBI): 90007
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Midbrain nucleolar protein (midnolin, MIDN) is abundantly expressed in embryonic midbrain and localizes in the nucleus and nucleolus. MIDN mRNA was strongly and abundantly expressed during early developmental stages, and that the expression was not limited to midbrain but also expressed in diverse adult mouse tissues, including heart, spleen, lung, liver, skeletal muscle, kidney and testis.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell Biol; CREB promotes beta cell gene expression by targeting Its coactivators to tissue-specific enhancers.; Authors - Sam Van de Velde
human	WB	Science; The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation; Authors - Xin Gu ; KO Validated
human	IHC,WB	Cancer Sci; Exosomal miR-222-3p contributes to castration-resistant prostate cancer by activating mTOR signaling; Authors - Weixi Wang
human	WB,IF	Biomedicines; Comprehensive Analysis Reveals Midnolin as a Potential Prognostic, Therapeutic, and Immunological Cancer Biomarker; Authors - Xin-Guo Zhang ; KD Validated
mouse	WB	J Cell Mol Med; Midnolin Correlates With Anti-Tumour Immunity and Promotes Liver Cancer Progression Through β-Catenin; Authors - Shaobo Huang
pig	WB	J Virol; Midnolin inhibits coronavirus proliferation by degrading viral proteins; Authors - Yahe Wang ; KD Validated