MIER1 Polyklonaler Antikörper

MIER1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 11452-1-AP

Synonyme

Er1, hMI ER1, hMIER1, hMi-er1, MI ER1

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  WB analysis of human testis using 11452-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11452-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11452-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11452-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11452-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11452-1-AP (MIER1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11452-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11452-1-AP (MIER1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11452-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11452-1-AP (MIER1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  FC experiment of A431 using 11452-1-AP

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MIER1 Polyclonal antibody (11452-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, HepG2-Zellen, Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

11452-1-AP bindet in WB, IHC, ChIP, ELISA MIER1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIER1 fusion protein Ag2010
• Vollständiger Name: mesoderm induction early response 1 homolog (Xenopus laevis)
• Berechnetes Molekulargewicht: 536 aa, 61 kDa
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC017423
• Gene symbol: MIER1
• Gene ID (NCBI): 57708
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,ChIP	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
mouse	IHC	Nat Commun; Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice; Authors - Yanhao Chen
human	WB	Cell Chem Biol; HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation; Authors - Yufeng Xiao