MIER1 Polyklonaler Antikörper

MIER1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11452-1-PBS

Synonyme

MIER 1, MI ER1, hMi-er1, hMIER1, hMI ER1

---

• 

  WB analysis of human testis using 11452-1-AP (same clone as 11452-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 11452-1-AP (same clone as 11452-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 11452-1-AP (same clone as 11452-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 11452-1-AP (same clone as 11452-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 11452-1-AP (same clone as 11452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11452-1-AP (MIER1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 11452-1-AP (same clone as 11452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11452-1-AP (MIER1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 11452-1-AP (same clone as 11452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11452-1-AP (MIER1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 11452-1-AP (same clone as 11452-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MIER1 Polyclonal antibody (11452-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 11452-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

11452-1-PBS bindet in WB, IHC, Indirect ELISA MIER1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIER1 fusion protein Ag2010
• Vollständiger Name: mesoderm induction early response 1 homolog (Xenopus laevis)
• Berechnetes Molekulargewicht: 536 aa, 61 kDa
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC017423
• Gene symbol: MIER1
• Gene ID (NCBI): 57708
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.