MIER2 Rekombinanter Antikörper

MIER2 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241458D2

Kat-Nr. : 84216-1-PBS

Synonyme

241458D2, KIAA1193, Mesoderm induction early response protein 2, Mi-er2

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  WB analysis using 84216-1-RR (same clone as 84216-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84216-1-RR (MIER2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84216-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84216-1-RR (same clone as 84216-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MIER2 antibody (84216-1-RR, Clone: 241458D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84216-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01148-1

  Cytometric bead array standard curve of MP01148-1, MIER2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84216-2-PBS. Detection antibody: 84216-1-PBS. Standard: Ag11491. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP01148-2

  Cytometric bead array standard curve of MP01148-2, MIER2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84216-3-PBS. Detection antibody: 84216-1-PBS. Standard: Ag11491. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84216-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84216-1-RR against Human MIER2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84216-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA MIER2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MIER2 fusion protein Ag11491
• Vollständiger Name: mesoderm induction early response 1, family member 2
• Berechnetes Molekulargewicht: 545 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC028203
• Gene symbol: MIER2
• Gene ID (NCBI): 54531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mesoderm induction early response 2 (MIER2) is a member of the MIER family of proteins, which is relatively conservative in evolution and includes three members: MIER1, MIER2, and MIER3. The MIER family is known to contribute to histone deacetylase (HDAC) activity and actively participate in histone deacetylation (PMID: 28046085; PMID: 38702028). In renal cell carcinoma, MIER2 represses PGC1A expression by recruiting HDAC1 to deacetylate P53 (PMID: 28046085).